Affinity chromatography: Difference between revisions
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'''Affinity chromatography''' (also called bioaffinity chromatography) is a method to filter a specific molecule out of a solution. | '''Affinity chromatography''' (also called bioaffinity chromatography) is a method to filter a specific [[molecule]] out of a solution. | ||
An affinity absorbent which consists of an immobilized molecule which can bond to the target molecule is used to immobilize the target molecule. | |||
==The process== | ==The process== | ||
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2. The molecules that bond bind get washed out. | 2. The molecules that bond bind get washed out. | ||
3. The affinity absorbed get seperated from the target molecule by either changing the pH value of the solution, ionic strength or polarity. <ref>[http://uuhsc.utah.edu/coe/hematology/protein/affinity_chromatography.pdf| Affinity Chromatography Principles and Methods from Amersham Biosciences]</ref> | 3. The affinity absorbed get seperated from the target molecule by either changing the [[pH]] value of the solution, ionic strength or polarity. <ref>[http://uuhsc.utah.edu/coe/hematology/protein/affinity_chromatography.pdf| Affinity Chromatography Principles and Methods from Amersham Biosciences]</ref> | ||
There are a variety of affinity absorbents which work for different molecules. It is important to select one that doesn't bond to other molecules in the solution. | There are a variety of affinity absorbents which work for different molecules. It is important to select one that doesn't bond to other molecules in the solution. | ||
Revision as of 20:27, 11 February 2009
Affinity chromatography (also called bioaffinity chromatography) is a method to filter a specific molecule out of a solution. An affinity absorbent which consists of an immobilized molecule which can bond to the target molecule is used to immobilize the target molecule.
The process
1. The affinity absorbent is added to the solution.
2. The molecules that bond bind get washed out.
3. The affinity absorbed get seperated from the target molecule by either changing the pH value of the solution, ionic strength or polarity. [1]
There are a variety of affinity absorbents which work for different molecules. It is important to select one that doesn't bond to other molecules in the solution.