Affinity chromatography: Difference between revisions
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imported>Christian Kleineidam (New page: Affinity chromatography (also called bioaffinity chromatography) is a method to filter a specific molecule out of a solution. A affinity absorbent which consists of an immobilized molecule...) |
imported>Christian Kleineidam No edit summary |
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==The process== | ==The process== | ||
1. The affinity absorbent is added to the solution. | 1. The affinity absorbent is added to the solution. | ||
2. The | |||
2. The molecules that bond bind get washed out. | |||
3. | |||
There are a variety of affinity absorbents which work for different molecules. It is important to select one that doesn't bond to other molecules in the solution. | There are a variety of affinity absorbents which work for different molecules. It is important to select one that doesn't bond to other molecules in the solution. | ||
==Sources== | |||
[http://uuhsc.utah.edu/coe/hematology/protein/affinity_chromatography.pdf| Affinity Chromatography Principles and Methods from Amersham Biosciences | |||
Revision as of 10:38, 19 March 2008
Affinity chromatography (also called bioaffinity chromatography) is a method to filter a specific molecule out of a solution. A affinity absorbent which consists of an immobilized molecule which can bond to the target molecule is used to immobilize the target molecule.
The process
1. The affinity absorbent is added to the solution.
2. The molecules that bond bind get washed out.
3.
There are a variety of affinity absorbents which work for different molecules. It is important to select one that doesn't bond to other molecules in the solution.
Sources
[http://uuhsc.utah.edu/coe/hematology/protein/affinity_chromatography.pdf%7C Affinity Chromatography Principles and Methods from Amersham Biosciences