Mycobacterium tuberculosis
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| Scientific classification | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ||||||||||||||
| Binomial name | ||||||||||||||
| Mycobacterium tuberculosis |
Description and significance
Describe the appearance, habitat, etc. of the organism, and why it is important enough to have its genome sequenced. Describe how and where it was isolated. Include a picture or two (with sources) if you can find them.
Genome structure
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Does it have any plasmids? Are they important to the organism's lifestyle?
- Double stranded circular DNA.
Chart from "http://genolist.pasteur.fr/TubercuList"
| DNA Molecule Summary | ||
| Total Number of all DNA molecules: | 1 | 100.00% |
| Total Size of all DNA molecules: | 4403837 bp | 100.00% |
| Number of Primary Annotation coding bases: | 4069477 bp | 92.40% |
| Number of G+C bases: | 2889216 bp | 65.60% |
| Primary Annotation Summary | ||
| Total genes: | 4294 | 100.00% |
| Protein coding genes: | 4246 | 98.88% |
| Genes assigned a role category: | 2317 | 54.56% |
| Genes not assigned a role category: | 2 | 0.04% |
| Conserved hypothetical genes: | 655 | 15.42% |
| Hypothetical genes: | 1272 | 29.95% |
| tRNA genes: | 45 | 1.04% |
| rRNA genes: | 3 | 0.06% |
Cell structure and metabolism
(Temporary General Outline)
- Obligate Aerobe: Explains why it infects the upper lobes of the lungs
- Slow rate of growth compared to other bacteria (about 15-20 hours)
- Non-motile rod shaped
- Size: ~2-4 um in length and 0.2-0.5 um in width
- Acid-fast staining is used (specifically Ziehl-Neelsan Stain)
- Cell wall: peptidoglycan but note 60% lipid (Mycolic Acid, Cord Factor, Wax-D) High lipid percentage results in a lot of interesting properties: (impermeability to stain; different kinds of resistances (antibiotics, osmotic lysis, acidic and alkaline compounds, oxidation).
Ecology
Describe any interactions with other organisms (included eukaryotes), contributions to the environment, effect on environment, etc.
Pathology
(Temporary General Outline)
- Major cause of tuberculosis (can link to tuberculosis page later)
- Binding to mannose receptors (for entry into cell)
- Ability to prevent lysosome from fusing with phagosome, as a result prevents the destruction of the bacteria.
- Slow generation may actually help the bacteria from being detected by the immune system.
- Cell wall construction is 60% lipid. Lot of different types of resistances.
- Antigen 85: binds to fibronectin and may help in protecting the bacteria from the immune system.
- Interference with oxygen intermediates
Application to Biotechnology
Does this organism produce any useful compounds or enzymes? What are they and how are they used?
Current Research
Summaries to come
Pubmed article about BCG vaccine
Pubmed article about the discovery of lipolytic enzymes which may be involved with virulence
Article concering multi-drug resistant mycobacterium tuberculosis