Fluorescent antibody technique: Difference between revisions
imported>Robert Badgett (New page: {{subpages}} In medicine and immunohistochemistry, '''fluorescent antibody technique''' ('''immunofluorescence''') is a immunologic test for "tissue antigen using either a ...) |
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===Indirect fluorescent antibody technique=== | ===Indirect fluorescent antibody technique=== | ||
Indirect fluorescent antibody technique is "a form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody."<ref>{{MeSH|Indirect fluorescent antibody technique}}</ref> | |||
==References== | ==References== | ||
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Revision as of 14:53, 18 October 2009
In medicine and immunohistochemistry, fluorescent antibody technique (immunofluorescence) is a immunologic test for "tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (fluorescent antibody technique, direct) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (fluorescent antibody technique, indirect). The tissue is then examined by fluorescence microscopy."[1]
Classification
Direct fluorescent antibody technique
Indirect fluorescent antibody technique
Indirect fluorescent antibody technique is "a form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody."[2]
References
- ↑ Anonymous (2024), Fluorescent antibody technique (English). Medical Subject Headings. U.S. National Library of Medicine.
- ↑ Anonymous (2024), Indirect fluorescent antibody technique (English). Medical Subject Headings. U.S. National Library of Medicine.