Induced pluripotent stem cells: Difference between revisions
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==Introduction== | ==Introduction== | ||
'''Induced pluripotent stem cells''' ('''iPS''' cells) are a class of [[stem cells]] that are artificially derived from differentiated embryonic or adult tissues. Therapeutically, iPS cells hold the potential to be used to treat a patient with their own cells (i.e. bone marrow transplant, spinal cord injury repair). | |||
Multiple strategies to generate iPS cells are being developed. | Multiple strategies to generate iPS cells are being developed. | ||
*Somatic cell nuclear transfer | *Somatic cell nuclear transfer | ||
*Embryonic stem cell fusion | *Embryonic stem cell fusion | ||
*Nuclear | *Nuclear reprogramming | ||
==Nuclear | ==Nuclear reprogramming== | ||
Based on | Based on previous studies, one research group set out to determine which genes are required to be expressed in order to generate/maintain embryonic stem cells.1 In a series of experiments it was determined that 4 factors are required to cause mouse fibroblasts to "dedifferentiate" into a stem-cell like state. | ||
*Sox2 | *Sox2 | ||
*Klf4 | *Klf4 | ||
| Line 14: | Line 16: | ||
*c-myc | *c-myc | ||
== | ==References== | ||
1. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006 Aug 25;126(4):663-76. Epub 2006 Aug 10. PMID: 16904174 [PubMed - indexed for MEDLINE] | 1. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006 Aug 25;126(4):663-76. Epub 2006 Aug 10. PMID: 16904174 [PubMed - indexed for MEDLINE] | ||
Latest revision as of 17:23, 26 December 2007
Introduction
Induced pluripotent stem cells (iPS cells) are a class of stem cells that are artificially derived from differentiated embryonic or adult tissues. Therapeutically, iPS cells hold the potential to be used to treat a patient with their own cells (i.e. bone marrow transplant, spinal cord injury repair).
Multiple strategies to generate iPS cells are being developed.
- Somatic cell nuclear transfer
- Embryonic stem cell fusion
- Nuclear reprogramming
Nuclear reprogramming
Based on previous studies, one research group set out to determine which genes are required to be expressed in order to generate/maintain embryonic stem cells.1 In a series of experiments it was determined that 4 factors are required to cause mouse fibroblasts to "dedifferentiate" into a stem-cell like state.
- Sox2
- Klf4
- Oct3/4
- c-myc
References
1. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006 Aug 25;126(4):663-76. Epub 2006 Aug 10. PMID: 16904174 [PubMed - indexed for MEDLINE]